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    西南大学发表文章中用zeta life转染试剂高效率转染HT-29细胞

    发布时间: 2021-07-23  点击次数: 306次

    西南大学用zeta life转染试剂高效率转染HCT116、SW480、HT29人结直肠癌细胞,其发表文章已见刊。

    西南大学生命科学学院教育部创新重点实验室

    使用zeta life,Advanced DNA RNA转染试剂

    高效率转染HCT-116, SW480, HT-29人结直肠癌细胞


    发表文章转染条件

    A、HCT-116, SW480, HT-29人结直肠癌细胞

    B、AURKA质粒

    C、转染细胞融合度50%

    D、96孔板每孔使用0.5μg质粒DNA

    6孔每孔使用10 ug质粒DNA

    (注意:本实验中用到的细胞密度、转染质粒DNA用量不适用于LIPO3000/2000)


    发表文章部分内容

    Overexpression of AURKA Plasmid for AURKA overexpression was obtained from VectorBuilder. Details about plasmid containing the AURKA gene can be found at . colon cancer cells (HCT-116, SW480, HT-29) were seeded at 50% confluency for 24 h in six-well and 96-well plates. Plasmid (10 ug per-well for six-well and 0.5 μg per-well for 96 plates) and the Advanced DNA RNA transfectionzeta life,USA) reagent were mixed in equal proportionsand added to the cell culture medium for 6 h. DOX (1 μg/ml) was added to induce the expression of AURKA. After addition of DOX and PAL for 36 h, cell viability was measured by MTT and apoptosis was detected by。

    西南大学文章节选.jpg

    PAL exerts anti-colon cancer effects by targeting AURKA. (A) PAL promotes apoptosis in colon cancer cells (HCT-116, SW480) in a dose-dependent manner. (B) PAL promotes G2/M phase arrest in colon cancer cells (HCT-116, SW480) in a dose-dependent manner. (C) The expression of AURKA after DOX treatment for 24 h. (D) Cell density after PAL treatment for 24 h in the cells (HCT-116, SW480) transfected with AURKA overexpression plasmids. DOX is an inducer of AURKA expression in plasmids. (E) Effect of PAL detected by MTT assay on the proliferation ability of colon cancer cells transfected with AURKA overexpression plasmids. (F) Effects of PAL detected by.


    深圳市安培生物科技有限公司是美国ZETA LIFE的中国深圳地区总代,我司代理其Advanced系列高效DNA、RNA转染试剂。在细胞转染实验中均有高效转染率,我司目前现货供应,助力每个科研人实现科研梦。公司的宗旨:致力成为推动生命科学进步的和值得信赖的合作者。

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